| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab181179-10μl |
10μl |
现货  |
| |
| Ab181179-50μl |
50μL |
现货 |
| |
| Ab181179-100μl |
100μL |
现货 |
| |
| Ab181179-1ml |
1ml |
期货 |
|
| 产品名称 | PCNA Mouse mAb |
|---|---|
| 别名 | PCNA小鼠单克隆抗体 | PCNA小鼠单抗 |
| 英文别名 | Cyclin | PCNA |
| 规格或纯度 | ExactAb™, Validated, Carrier Free, Azide Free, High performance, 0.5 mg/mL |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | PCNA |
| 种属反应性 | 人(Human),大鼠(Rat),小鼠(Mouse) |
| 预测反应种属 | 兔(Rabbit),牛(Cow),狗(Dog),猪(Pig),鸡(Chicken) |
| 免疫原 | Full length recombinant human PCNA |
| 阳性对照 | WB: HeLa, A431, HEK293, HepG2, NIH3T3 and C6 whole cell lysates Flow: HeLa cells |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| 克隆号 | C12/17A3 |
| Format | Whole IgG |
| 亚型 | IgG2a |
| 轻链亚型 | kappa |
| SDS-PAGE | 185 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | PBS, pH 7.2 |
| 防腐剂 | No |
| 浓度 | 0.5 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 短期储存（1-2 周）。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
PCNA Mouse mAb (Ab181179) - Western Blot
All lanes: PCNA Antibody (Ab181179) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 29.0 kDa
Observed band size: 31.0 kDa
Exposure time: 54.2 s
PCNA Mouse mAb (Ab181179) - Flow Cytometry
Overlay histogram showing HeLa cells stained with PCNA Mouse mAb (Ab181179) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
| 应用名称 | 稀释比例 |
|---|---|
| WB |
1/1,000-1/10,000 ; Predicted molecular weight: 29 kDa.
|
| Flow Cytometry | 0.032 μg/mL - 1.0 μg/mL |
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| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ24F0102682 |
分析证书 | 24-02-01 | Ab181179 |
| ZJ24F0102681 |
分析证书 | 24-02-01 | Ab181179 |
| ZJ24F0102680 |
分析证书 | 24-02-01 | Ab181179 |
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| 23. Unk, Ildiko I and 7 more authors.. (2008) Human HLTF functions as a ubiquitin ligase for proliferating cell nuclear antigen polyubiquitination.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:18316726] |
| 24. Farina, Andrea A and 6 more authors.. (2008) Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1.. The Journal of biological chemistry, (25): [PMID:18499658] |
| 25. Nishitani, Hideo H and 5 more authors.. (2008) CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation.. The Journal of biological chemistry, (24): [PMID:18703516] |
| 26. Motegi, Akira A and 10 more authors.. (2008) Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:18719106] |
| 27. Abbas, Tarek T and 5 more authors.. (2008) PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex.. Genes & development, (15): [PMID:18794347] |
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| 29. Yu, Yu Y and 13 more authors.. (2009) Proliferating cell nuclear antigen is protected from degradation by forming a complex with MutT Homolog2.. The Journal of biological chemistry, (17): [PMID:19419956] |
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| 33. Ogi, Tomoo T and 13 more authors.. (2010) Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells.. Molecular cell, (12): [PMID:20227374] |
| 34. De Haro, Leyma P LP and 9 more authors.. (2010) Metnase promotes restart and repair of stalled and collapsed replication forks.. Nucleic acids research, [PMID:20457750] |
| 35. Groehler, Angela L AL and Lannigan, Deborah A DA.. (2010) A chromatin-bound kinase, ERK8, protects genomic integrity by inhibiting HDM2-mediated degradation of the DNA clamp PCNA.. The Journal of cell biology, (23): [PMID:20733054] |
| 36. Rowbotham, Samuel P SP and 12 more authors.. (2011) Maintenance of silent chromatin through replication requires SWI/SNF-like chromatin remodeler SMARCAD1.. Molecular cell, (6): [PMID:21549307] |
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