基本信息

产品名称	Goat Anti-Mouse IgG H&L (Biotin)
别名	山羊抗小鼠IgG抗体 (Biotin) \| 山羊抗小鼠IgG (Biotin) \| 羊抗小鼠IgG抗体 (Biotin) \| 羊抗小鼠IgG (Biotin) \| 山羊抗小鼠IgG H&L 抗体 (Biotin) \| 山羊抗小鼠IgG H&L (Biotin) \| 羊抗小鼠IgG H&L 抗体 (Biotin) \| 羊抗小鼠IgG H&L (Biotin) \| 山羊抗鼠 IgG H&L(生物素)
英文别名	Goat Anti-Mouse IgG H&L Antibody (Biotin) \| Anti-Mouse IgG H&L Antibody (Biotin) \| Mouse IgG H&L Antibody (Biotin) \| Biotin-conjugated Goat Anti-Mouse IgG (H+L) \| Goat Anti Mouse IgG Antibody (Biotin) \| Anti-Mouse IgG Antibody (Biotin) \| Mouse IgG Antibod
规格或纯度	ExactAb™, 高性能, 已验证, 无叠氮钠, 1.0 mg/mL
宿主种属	山羊(Goat)
种属反应性	小鼠(Mouse)
交叉反应	Reacting with mouse whole IgG (mouse IgG light chain, heavy chain), may cross react with immunoglobulins from other species.
免疫原	Mouse IgG
偶联	Biotin

AI解读

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Goat IgG
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified
物理外观	Liquid
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	1.0 mg/mL
储存温度	2-8°C储存,避光,避免反复冻融
运输条件	冰袋运输
稳定性与储存	在 4°C 下可保存一年。避免反复冻融循环。避免光照。
分子类型	抗体

图片

IF: Goat Anti-Mouse IgG H&L (Biotin) (Ab179002)

Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Streptavidin protein (FITC) (rp155876) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Western Blot: Goat Anti-Mouse IgG H&L (Biotin) (Ab179002)

Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at 1/20000 dilution
SA-HRP: 1:20000

Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 67s

Flow Cytometry: Goat Anti-Mouse IgG H&L (Biotin) (Ab179002)

Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/300 was used as the secondary antibody. Then incubated with Streptavidin Protein (FITC) (rp155876) at a dilution of 1/1000. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

ELISA: Goat Anti-Mouse IgG H&L (Biotin) (Ab179002)

Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - ELISA
Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) was tested by direct ELISA. The plate was coated with different amounts of Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.