货号 (SKU)

包装规格

是否现货

价格

数量

S665689-200gels

200-300gels

期货

S665689-40gels

40-60gels

现货

基本描述

英文名称

SDS-PAGE Gel Kit

储存温度

2-8°C储存

运输条件

冰袋运输

产品介绍

产品内容

S665689	Component	40-60gels	200-300gels	Storage
S665689A	30%Acr-Bis (29:1)	100 mL	2×250 mL	2-8℃
S665689B	SDS-PAGE Separating Gel Buffer (4×)	100 mL	500 mL	2-8℃
S665689C	SDS-PAGE Stacking Gel Buffer (4×)	50 mL	250 mL	2-8℃
S665689D	APS	0.5 g	2.5 g	2-8℃
S665689E	TEMED	1 mL	5 mL	2-8℃

本产品所提供的APS（过硫酸铵）为固体粉末，使用前加入纯水溶解即配制成10%APS溶液（0.5 g APS加5 ml纯水、2.5 g APS加25 ml纯水），将溶液分装后置于-20℃保存，通常半年内有效。溶液在使用中可放置4℃保存两周。

产品简介

本产品包括SDS-PAGE凝胶制备所需全套试剂，只需自备纯水，即可制备高质量各种浓度的变性PAGE凝胶，方便、快捷。本试剂盒在分离胶缓冲液和浓缩胶缓冲液中已加入10%SDS，使用时不用另外加入。

注意事项

1. 10%APS配制后分装-20度保存。APS溶液不稳定，应尽量减少室温存放时间，每次取用后立即放回冰箱，以防失效；若发现凝胶聚合时间延长，应考虑更换使用

-20度保存的10%APS。

2. PAGE凝胶的凝聚速度与温度以及APS、TEMED的用量密切相关；在其它条件不变的情况下，可通过改变APS及TEMED的用量，控制PAGE凝胶的聚合速度，凝胶聚合过快不利于操作；附表中的APS及TEMED量可供参考，应根据实际操作情况做适当的调整。

3. 在凝胶配制过程中，尤其是液体混匀步骤，应尽量避免气泡的产生。

4. 在分离胶上层加纯水时要小心操作，加水时速度不能太快。

5. 丙烯酰胺具有神经毒性，操作时请穿着实验服并佩戴一次性手套。

6. 本产品仅用于科研，不能用于人体实验或人体治疗。

操作步骤

根据目的蛋白分子量大小选择合适的PAGE分离胶配制浓度，最佳胶浓度请参考附表1。

I灌制分离胶(各试剂使用量请参考附表3)

1．参照凝胶模具说明书，装配好凝胶模具。

注：加入上层筛板有助于加样时保持填料与样品均匀接触，是否加入上层筛板可根据实际情况选择。

2. 将不同体积的30%Acr-Bis(29:1)、SDS-PAGE Separating Gel Buffer和纯水在小烧杯或试管中混合。

3. 加入10%APS和TEMED，轻轻搅拌使其混匀，避免产生气泡。

4. 在凝胶模具中灌入适量分离胶溶液（对于mini-gel，凝胶液加至约距前玻璃板顶端

1.5 cm或距梳齿约0.5 cm即可），然后在分离胶溶液上轻轻覆盖一层1 cm的水层，使凝胶表面保持平整。

5. 静置30-60分钟，待分离胶和水层之间出现一个清晰的界面后，表面凝胶已聚合。

II 灌制浓缩胶（各试剂使用量请参考附表2）

1. 去除覆盖在分离胶上的水层。

2. 将不同体积的30%Acr-Bis(29:1)、SDS-PAGE Stacking Gel Buffer和纯水在一个小烧杯或试管中混合。

3. 加入10%APS和TEMED，轻轻搅拌使其混匀，避免产生气泡。

4. 将浓缩胶溶液加至分离胶的上面，直至凝胶溶液到达前玻璃板的顶端。

5. 将梳子插入凝胶内，避免产生气泡。

6. 静置10~20分钟，等待浓缩胶聚合。

7. 待凝胶聚合后，小心地拔出梳子，以免破坏加样孔。

8. 进行常规电泳操作。

附表

附表1. SDS-PAGE分离胶的浓度与最佳分离范围

附表2. 配制5%SDS-PAGE浓缩胶

附表3. 配制SDS-PAGE分离胶

Product content

S665689	Component	40-60gels	200-300gels	Storage
S665689A	30%Acr-Bis (29:1)	100 mL	2×250 mL	2-8℃
S665689B	SDS-PAGE Separating Gel Buffer (4×)	100 mL	500 mL	2-8℃
S665689C	SDS-PAGE Stacking Gel Buffer (4×)	50 mL	250 mL	2-8℃
S665689D	APS	0.5 g	2.5 g	2-8℃
S665689E	TEMED	1 mL	5 mL	2-8℃

The APS (Ammonium Persulfate) supplied with this product is a solid powder, which is dissolved in purified water before use to form a 10% APS solution (0.5 g APS plus 5 ml of purified water, 2.5 g APS plus 25 ml of purified water), and the solution is dispensed and placed at -20°C for storage, which is usually effective for six months. The solution can be placed in use and stored at 4°C for two weeks.

Products

This product includes a full set of reagents required for the preparation of SDS-PAGE gels, only need to prepare their own pure water, you can prepare high quality denaturing PAGE gels of various concentrations, convenient and fast. 10% SDS has been added to the separation buffer and concentration buffer, so there is no need to add SDS to the buffer.

Caveat

1.10% APS is prepared and stored at -20 degrees C. APS solution is unstable and should be stored at room temperature for as little time as possible, and returned to the refrigerator immediately after each use to prevent failure; if the gel polymerization time is found to be prolonged, consideration should be given to replacing it.

-10% APS kept at 20 degrees.

2. The cohesion speed of PAGE gel is closely related to the temperature and the dosage of APS and TEMED; in the case of other conditions remain unchanged, the polymerization speed of PAGE gel can be controlled by changing the dosage of APS and TEMED, and the gel polymerization is too fast for operation; the amount of APS and TEMED in the attached table can be used for reference, and should be adjusted appropriately according to the actual operation.

3. During the gel preparation process, especially the liquid mixing step, the generation of air bubbles should be avoided as much as possible.

4. Be careful when adding pure water to the upper layer of the separation gel, and do not be too fast when adding water.

5. Acrylamide is neurotoxic, please wear lab coat and disposable gloves when handling.

6. This product is for scientific research only and cannot be used for human experimentation or human treatment.

Procedure

According to the molecular weight size of the target protein, select the appropriate concentration of PAGE separation gel preparation, the optimal gel concentration, please refer to Exhibit 1.

I Infusion of separating gel (please refer to Exhibit 3 for the amount of each reagent used)

1. Refer to the gel mold instructions and assemble the gel mold.

Note: The addition of the upper sieve plate helps to maintain uniform contact between the filler and the sample when adding samples, and the addition of the upper sieve plate can be selected according to the actual situation.

2. Mix different volumes of 30% Acr-Bis (29:1), SDS-PAGE Separating Gel Buffer and pure water in a small beaker or test tube.

3. Add 10% APS and TEMED, stir gently to mix well and avoid air bubbles.

4. Fill the gel mold with the appropriate amount of separator gel solution (for mini-gel, add gel solution to about the top of the front glass plate).

(1.5 cm or about 0.5 cm from the comb teeth is sufficient), and then gently cover the separating gel solution with a 1 cm layer of water to keep the gel surface flat.

5. Let it stand for 30-60 minutes, after a clear interface appears between the separated gel and the water layer, the surface gel has been polymerized.

II Filling of concentrated gel (please refer to Exhibit 2 for the amount of each reagent used)

1. Remove the water layer covering the separator gel.

2. Mix different volumes of 30% Acr-Bis (29:1), SDS-PAGE Stacking Gel Buffer and pure water in a small beaker or test tube.

3. Add 10% APS and TEMED, stir gently to mix well and avoid air bubbles.

4. Add the concentrated gel solution to the top of the separation gel until the gel solution reaches the top of the front glass plate.

5. Insert the comb into the gel to avoid air bubbles.

6. Let it stand for 10~20 minutes and wait for the concentrated gel to polymerize.

7. After the gel has polymerized, carefully pull out the comb so as not to damage the spiking hole.

8. Perform routine electrophoresis operations.

Schedules

Exhibit 1. Concentration and optimal separation range of SDS-PAGE separation gel

SDS-PAGE separation gel concentration	Optimal separation range
6% glue	50-150 kD
8% glue	30-90 kD
10% glue	20-80 kD
12% glue	12-60 kD
15% glue	10-40 kD

Schedule 2. Preparation of 5% SDS-PAGE gel concentrate

Gel volume	Required volume of each component (unit: ml)
	pure water	30%Acr-Bis(29:1)	SDS-PAGE Stacking Gel Buffer（4×）	10%APS	TEMED
2ml	1.14	0.34	0.5	0.02	0.002
4ml	2.28	0.68	1	0.04	0.004
6ml	3.42	1.02	1.5	0.06	0.006
8ml	4.56	1.36	2.0	0.08	0.008

Schedule 3. Preparation of SDS-PAGE Separation Gel

Separation gel concentration	Gel volume	Required volume of each component (unit: ml)
		pure water	30%Acr-Bis(29:1)	SDS-PAGE Separating Gel Buffer（4×）	10%APS	TEMED
6%	5ml	2.75	1.0	1.25	0.05	0.004
	10ml	5.5	2.0	2.5	0.1	0.008
	15ml	8.25	3.0	3.75	0.15	0.012
	20ml	11	4.0	5	0.2	0.016
8%	5ml	2.42	1.33	1.25	0.05	0.003
	10ml	4.8	2.7	2.5	0.1	0.006
	15ml	7.25	4.0	3.75	0.15	0.009
	20ml	9.7	5.3	5	0.2	0.012
10%	5ml	2.08	1.67	1.25	0.05	0.002
	10ml	4.17	3.33	2.5	0.1	0.004
	15ml	6.25	5.0	3.75	0.15	0.006
	20ml	8.3	6.7	5	0.2	0.008
12%	5ml	1.75	2.0	1.25	0.05	0.002
	10ml	3.5	4.0	2.5	0.1	0.004
	15ml	5.25	6.0	3.75	0.15	0.006
	20ml	7.0	8.0	5	0.2	0.008
15%	5ml	1.25	2.5	1.25	0.05	0.002
	10ml	2.5	5.0	2.5	0.1	0.004
	15ml	3.75	7.5	3.75	0.15	0.006
	20ml	5	10.0	5	0.2	0.008

AI解读

安全和危险性（GHS）

SDS英文版

技术规格说明书

Physicochemical Property	pass
Performance	Pass
Appearance(S665689A)	Liquid
Appearance(S665689B)	Liquid
Appearance(S665689C)	Liquid
Appearance(S665689D)	solid
Appearance(S665689E)	Liquid

技术规格说明书

s665689_sds-page gel kit.pdf

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到2个结果

批号(Lot Number)	证书类型	日期	货号
L2402652	分析证书	24-11-21	S665689
E2427212	分析证书	24-05-28	S665689

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稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

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复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

SDS-PAGE凝胶试剂盒

库存信息

库存信息

基本描述

AI解读

安全和危险性（GHS）

技术规格说明书

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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