| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| S665584-100T |
100T |
期货  |
| |
| S665584-500T |
500T |
期货 |
|
| 别名 | 超级 DNA 标记 | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 英文名称 | Super DNA Marker | ||||||||||||||
| 储存温度 | -20°C储存,避免反复冻融 | ||||||||||||||
| 运输条件 | 超低温冰袋运输 | ||||||||||||||
| 产品介绍 |
产品内容
产品简介 Super DNA Marker由10条DNA片段组成，分别为10,000 bp、5,000 bp、3,000 bp、 2,000 bp、1,500 bp、1,000 bp、750 bp、500 bp、250 bp和100 bp。本产品为已含 有1×Loading Buffer的DNA溶液，可取5 μl直接电泳，使用十分方便；电泳时1,000 bp 的DNA片段量约为150 ng，显示亮带，其他条带的DNA量约为50 ng。 实验前准备及重要注意事项 1．本产品可直接化冻混匀使用，无需加热。 2．请及时更换电泳缓冲液并使用新制琼脂糖凝胶，以免影响电泳结果。 3．DNA琼脂糖凝胶电泳时，凝胶的纯度对DNA条带的清晰度影响很大，因此尽量选用 质量好的琼脂糖，推荐凝胶浓度为1%-3%，电压4-8 v/cm。 4．凝胶浓度与DNA片段的分离性能关系密切。凝胶浓度越大，对短片段DNA分离性能 越好；反之，凝胶浓度越小，越有利于长片段DNA的分离。 5．当与大分子染料配合使用时，建议适当减少Marker的用量或加大染料的用量。 使用方法 取5 μl加入琼脂糖凝胶的加样孔中（如果加样孔较宽，大于6 mm时，须适当增加上 样量），进行电泳。 实验结果
1%琼脂糖凝胶电泳图(5 μl)
Product content
Product Introduction Super DNA Marker consists of 10 DNA fragments, 10,000bp, 5,000bp, 3,000bp, 2,000bp, 1,500bp, 1,000bp, 750bp, 500bp, 250bp, and 100bp respectively, and it can be directly electrophoresed with 5μl of 1×Loading Buffer. This product is a DNA solution containing 1×Loading Buffer, 5μl of which can be taken for direct electrophoresis, which is very convenient to use; the amount of DNA fragment of 1,000bp in electrophoresis is about 150ng, which shows a bright band, and the amount of DNA in other bands is about 50ng. Pre-experiment Preparation and Important Notes 1. This product can be directly frozen and mixed for use without heating. 2. Please change the electrophoresis buffer and use fresh agarose gel in time to avoid affecting the electrophoresis results. 3. When DNA agarose gel electrophoresis, the purity of the gel has a great influence on the clarity of the DNA bands, so try to use good quality agarose, the recommended gel concentration is 1%-3%, voltage 4-8v/cm. 4. The gel concentration is closely related to the separation performance of DNA fragments. The larger the gel concentration, the better the separation performance of short fragment DNA; conversely, the smaller the gel concentration, the more favorable the separation of long fragment DNA. 5. When used with macromolecular dyes, it is recommended to appropriately reduce the amount of Marker or increase the amount of dye. Usage Take 5 μl into the spiking well of the agarose gel (if the spiking well is wider than 6 mm, the amount of sample must be increased appropriately), and carry out electrophoresis.
1% agarose gel electropherogram (5 μ L) |
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