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彩色预染蛋白分子量标准 (2.7-40 kDa)

  • 2.7-40 kDa
    •
  • 2.7 Blue/ 4.2 Blue/ 8 Green/ 15 Blue/ 25 Blue/ 40 Orange kDa
功能和特点
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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
rp192286-250μl
 250μL 现货 Stock Image
rp192286-4×250μl
 4×250μl 现货 Stock Image
大包装询价 添加到收藏夹 比较  DATASHEET
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蛋白质分子量标准品 (5) 预染蛋白Marker (5)
main product photo

基本描述

产品名称 彩色预染蛋白分子量标准 (2.7-40 kDa)
别名 三色预染蛋白Marker | 多色低分子量蛋白分子量标准 | 彩色预染蛋白分子量标准 | 蛋白Ladder
英文别名 Prestained Color Protein Marker | Prestained Color Protein Ladder | Broad Multi Color Pre-Stained Protein Standard | Star Ribbon Pre-stained Protein Marker | Tricolor Pre-Stained Protein Marker | Protein Ladder
规格或纯度 2.7-40 kDa, 2.7 Blue/ 4.2 Blue/ 8 Green/ 15 Blue/ 25 Blue/ 40 Orange kDa
产品介绍

彩色预染蛋白分子量标准：

1. 该产品包含了从2.7kDa到40kDa共6种高度纯化并预染的重组蛋白质。

2. 适合作为SDS-PAGE和Western的蛋白质分子量标准。

3. 本彩色预染蛋白质分子量标准已经配制在1×SDS-PAGE上样缓冲液中，直接使用，不要煮沸、稀释和加入还原剂处理。

4. 根据上样孔的大小，本彩色预染蛋白质分子量标准通常每次上样5-10微升（5×1.5mm胶孔5-10ul足够），即可在电泳时、电泳后和转膜后观察到非常清楚的蛋白条带。

预染蛋白质分子量标准经非预染蛋白质分子量标准标定，具有如下突出优势：

1. 蓝色和绿色条带在Tris-Glycine，Bis-Tris，Tris-Acetate缓冲体系SDS-PAGE胶其表观分子量基本没有变化。

2. 橙(红)色条带与进口产品变化一致。

使用方法：

1. 室温下解冻后完全溶解并轻轻充分混匀，不要煮沸。

2. 取本产品5µl与实验样品同时进行聚丙烯酰胺凝胶电泳；建议有条件的实验室在初次使用本产品时可以根据自身的实验条件和实验习惯通过预实验确定合适的上样量，这样可以节约成本，同时获得效果更佳的实验图片。

3. 未使用的彩色预染蛋白分子量标准保存于储存条件，在4°C可放置2个月。

注意事项：

1. 注意电泳时间，低分子量蛋白可能会泳动出蛋白胶前沿。

2. Westernblot时需要注意小分子特殊性，特别对于小于3kDa的条带，容易穿膜而过。另外建议转膜液不添加SDS，若实验必须使用，使用SDS浓度不超过0.02%-0.04%。

3. 预染蛋白质在不同的缓冲体系下有不同的表观分子量，如果在该缓冲体系中事先用非预染蛋白质标定，可以大致确定蛋白质分子量。

Color pre dyed protein molecular weight standard:
1. This product contains six highly purified and pre stained recombinant proteins ranging from 2.7kDa to 40kDa.
2. Suitable as a protein molecular weight standard for SDS-PAGE and Western blotting.
3. This color pre stained protein molecular weight standard has been prepared in 1 × SDS-PAGE buffer and should be used directly without boiling, diluting, or adding reducing agents.
4. According to the size of the sample well, this color pre stained protein molecular weight standard usually requires 5-10 microliters of sample to be loaded each time (5-10μl for 5 × 1.5mm gel wells is sufficient), and very clear protein bands can be observed during electrophoresis, electrophoresis, and membrane transfer.

The pre stained protein molecular weight standard, calibrated with non pre stained protein molecular weight standards, has the following outstanding advantages:
1. The blue and green bands showed little change in apparent molecular weight on SDS-PAGE gel in Tris Glycine, Bis Tris, and Tris Acetate buffer systems.
2. The orange (red) color stripes are consistent with the changes in imported products. 

Usage method:
1. After thawing at room temperature, completely dissolve and gently mix well, do not boil.
2. Take 5µl of this product and test sample for polyacrylamide gel electrophoresis at the same time; It is recommended that laboratories with conditions determine the appropriate sample size through pre experiments based on their own experimental conditions and habits when using this product for the first time. This can save costs and obtain better experimental images.
3. Unused color pre dyed protein molecular weight standards should be stored under storage conditions and can be left at 4°C for 2 months.

Matters needing attention:
1. Pay attention to the electrophoresis time, as low molecular weight proteins may swim out of the protein gel front.
2. When Western blotting, attention should be paid to the specificity of small molecules, especially for bands smaller than 3kDa, which are prone to pass through the membrane. Additionally, it is recommended not to add SDS to the transfer solution. If it is necessary for the experiment, the concentration of SDS should not exceed 0.02% -0.04%.
3. Pre stained proteins have different apparent molecular weights in different buffer systems. If non pre stained proteins are calibrated in advance in this buffer system, the protein molecular weight can be roughly determined.
SDS-PAGE 2.7 Blue/ 4.2 Blue/ 8 Green/ 15 Blue/ 25 Blue/ 40 Orange kDa

AI解读

图片

Prestained Color Protein MW Marker (2.7-40 kDa) (rp192286) - SDS-PAGE band profile in Tris-Acetate
lmages are from a Tris-Acetate gel (SDS-PAGE) and subsequent transfer to membrane. Prestained Color Protein MW Marker (2.7-40 kDa) (rp192286) are mixture of 6 recombinant proteins (2.7-40 kDa), 4 blue-stained bands, a green-stained band (8 kDa) and an orange-stained band (40 kDa).

产品规格参数

物理形态 液体
储存缓冲液 20 mMTris-H3PO4, 3mM DTT, 2mM EDTA, 15% (W/V)Glycerol, 1.5% (W/V)SDS, 0.1% (V/V) Proclin300, 4M Urea, pH7.5
防腐剂 0.1% (V/V) Proclin 300
浓度 2.7 Blue/ 4.2 Blue/ 8 Green/ 15 Blue/ 25 Blue/ 40 Orange kDa
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store at 4℃ short term (2 months). Store at -20℃ long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

安全和危险性（GHS）

 SDS英文版

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批号(Lot Number) 证书类型 日期 货号
ZJ24F1114033 分析证书 24-11-19 rp192286

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