基本描述

产品名称

彩色预染蛋白分子量标准 (10-250kDa)

别名

三色预染蛋白Marker | 多色低分子量蛋白分子量标准 | 彩色预染蛋白分子量标准 | 蛋白Ladder

英文别名

规格或纯度

10-250kDa, 10 Blue/ 15 Blue/ 20 Blue/ 25 Green/ 30 Blue/ 40 Blue/ 50 Blue/ 72 Orange/ 100 Blue/ 130 Blue/ 250 Blue kDa

产品介绍

彩色预染蛋白分子量标准：

1. 该产品包含了从10kDa到250kDa共11种高度纯化并预染的重组蛋白质。

2. 适合作为SDS-PAGE和Western的蛋白质分子量标准。

3. 本彩色预染蛋白质分子量标准已经配制在1×SDS-PAGE上样缓冲液中，直接使用，不要煮沸、稀释和加入还原剂处理。

4. 根据上样孔的大小，本彩色预染蛋白质分子量标准通常每次上样5-10微升（5×1.5mm胶孔5ul足够），即可在电泳时、电泳后和转膜后观察到非常清楚的蛋白条带。

预染蛋白质分子量标准经非预染蛋白质分子量标准标定，具有如下突出优势：

1. 蓝色和绿色条带在Tris-Glycine，Bis-Tris，Tris-Acetate缓冲体系SDS-PAGE胶其表观分子量基本没有变化。

2. 橙(红)色条带与进口产品变化一致。

使用方法：

1. 室温下解冻后完全溶解并轻轻充分混匀，不要煮沸。

2. 取本产品5µl与实验样品同时进行聚丙烯酰胺凝胶电泳；建议有条件的实验室在初次使用本产品时可以根据自身的实验条件和实验习惯通过预实验确定合适的上样量，这样可以节约成本，同时获得效果更佳的实验图片。

3. 未使用的彩色预染蛋白分子量标准保存于储存条件，在4°C可放置2个月。

注意事项：

1. 注意电泳时间，低分子量蛋白可能会泳于染料前缘。

2. 大分子量蛋白Westernblot时需要延长转膜时间或加高转膜电压。另外建议转膜液不添加SDS，若实验必须使用，使用SDS浓度不超过0.02%-0.04%。

3. 预染蛋白质在不同的缓冲体系下有不同的表观分子量，如果在该缓冲体系中事先用非预染蛋白质标定，可以大致确定蛋白质分子量。

Color pre dyed protein molecular weight standard:
1. This product contains 11 highly purified and pre stained recombinant proteins ranging from 10kDa to 250kDa.
2. Suitable as a protein molecular weight standard for SDS-PAGE and Western blotting.
3. This color pre stained protein molecular weight standard has been prepared in 1 × SDS-PAGE buffer and should be used directly without boiling, diluting, or adding reducing agents.
4. According to the size of the sample well, this color pre stained protein molecular weight standard usually requires 5-10 microliters of sample to be loaded each time (5μl for 5 × 1.5mm gel wells is sufficient), and very clear protein bands can be observed during electrophoresis, electrophoresis, and membrane transfer.

The pre stained protein molecular weight standard, calibrated with non pre stained protein molecular weight standards, has the following outstanding advantages:
1. The blue and green bands showed little change in apparent molecular weight on SDS-PAGE gel in Tris Glycine, Bis Tris, and Tris Acetate buffer systems.
2. The orange (red) color stripes are consistent with the changes in imported products.

Usage method:
1. After thawing at room temperature, completely dissolve and gently mix well, do not boil.
2. Take 5µl of this product and test sample for polyacrylamide gel electrophoresis at the same time; It is recommended that laboratories with conditions determine the appropriate sample size through pre experiments based on their own experimental conditions and habits when using this product for the first time. This can save costs and obtain better experimental images.
3. Unused color pre dyed protein molecular weight standards should be stored under storage conditions and can be left at 4°C for 2 months.

Matters needing attention:
1. Pay attention to the electrophoresis time, as low molecular weight proteins may swim at the front edge of the dye.
2. During Western blotting of high molecular weight proteins, it is necessary to extend the transfer time or increase the transfer voltage. Additionally, it is recommended not to add SDS to the transfer solution. If it is necessary for the experiment, the concentration of SDS should not exceed 0.02% -0.04%.
3. Pre stained proteins have different apparent molecular weights in different buffer systems. If non pre stained proteins are calibrated in advance in this buffer system, the protein molecular weight can be roughly determined.

预测分子量

10-250KDa

SDS-PAGE

10 Blue/ 15 Blue/ 20 Blue/ 25 Green/ 30 Blue/ 40 Blue/ 50 Blue/ 72 Orange/ 100 Blue/ 130 Blue/ 250 Blue kDa

图片

SDS-PAGE band profile in Tris-Glycine: Prestained Color Protein MW Marker (10-250kDa) (rp192041)

Prestained Color Protein MW Marker (10-250kDa) (rp192041) - SDS-PAGE band profile in Tris-Glycine
lmages are from a Tris-glycine gel (SDS-PAGE) and subsequent transfer to membrane. Prestained Color Protein MW Marker (10-250kDa) (rp192041) are mixture of 11 recombinant proteins, 9 blue-stained bands, a green-stained band (25kDa) and an orange-stained band (72kDa).

SDS-PAGE band profile in Bis-Tris: Prestained Color Protein MW Marker (10-250kDa) (rp192041)

Prestained Color Protein MW Marker (10-250kDa) (rp192041) - SDS-PAGE band profile in Bis-Tris
lmages are from a Bis-Tris gel (SDS-PAGE) and subsequent transfer to membrane. Prestained Color Protein MW Marker (10-250kDa) (rp192041) are mixture of 11 recombinant proteins, 9 blue-stained bands, a green-stained band (25kDa) and an orange-stained band (65kDa).

Migration patterns in different electrophoretic conditions: Prestained Color Protein MW Marker (10-250kDa) (rp192041)

Prestained Color Protein MW Marker (10-250kDa) (rp192041) - Migration patterns in different electrophoretic conditions
The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein using the Prestained Color Protein MW Marker (10-250kDa) (rp192041) in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.

产品规格参数

物理形态	液体
储存缓冲液	20 mMTris-H3PO4, 3mM DTT, 2mM EDTA, 15% (W/V)Glycerol, 1.5% (W/V)SDS, 0.1% (V/V) Proclin300, 4M Urea, pH7.5
防腐剂	0.1% (V/V) Proclin 300
浓度	10 Blue/ 15 Blue/ 20 Blue/ 25 Green/ 30 Blue/ 40 Blue/ 50 Blue/ 72 Orange/ 100 Blue/ 130 Blue/ 250 Blue kDa
储存温度	-20°C储存
运输条件	超低温冰袋运输
稳定性与储存	Store at 4℃ short term (2 months). Store at -20℃ long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

安全和危险性（GHS）

SDS英文版

技术规格说明书

技术规格说明书

rp192041_ 彩色预染蛋白分子量标准 ,10-250 kda产品说明书.pdf

rp192041_ prestained color protein mw marker ,10-250 kda product manual.pdf

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到2个结果

批号(Lot Number)	证书类型	日期	货号
ZJ24F1114031	分析证书	24-11-19	rp192041
ZJ24F1113387	分析证书	24-11-06	rp192041

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

=

浓度

x

体积

x

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

x

体积 1 (V1)

=

浓度 2 (C2)

x

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

=

质量（小瓶）

÷

所需的复溶浓度

货号 (SKU)	包装规格	是否现货	价格	数量
rp192041-250μl	250μL	现货
rp192041-4×250μl	4×250μl	现货

彩色预染蛋白分子量标准 (10-250kDa)

库存信息

库存信息

库存信息

基本描述

图片

产品规格参数

安全和危险性（GHS）

技术规格说明书

质检证书（CoA,COO,BSE/TSE 和分析图谱)

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器