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基本描述
| 规格或纯度 | 胚胎细胞培养级 | ||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 稳定性与储存 | -20℃保存，至少一年有效。4℃保存，3个月内有效。 | ||||||||||||||||||||||||||||||||||||||||||
| 英文名称 | UltraBio™ Gelatin Solution | ||||||||||||||||||||||||||||||||||||||||||
| 储存温度 | -20°C储存 | ||||||||||||||||||||||||||||||||||||||||||
| 运输条件 | 超低温冰袋运输 | ||||||||||||||||||||||||||||||||||||||||||
| 产品介绍 |
UltraBio™ 0.1%明胶溶液（胚胎细胞培养级） 阿拉丁生产的UltraBio™ 0.1%明胶溶液，即UltraBio™ 0.1%Gelatin Solution，是一种经测试可以直接用于胚胎干细胞培养的无菌高品质0.1%明胶溶液。本产品主要用于细胞培养板、培养皿等表面的包被(Coating)，以促进哺乳动物细胞的贴壁和增殖。 明胶是细胞外基质蛋白胶原(Collagen)经过不可逆变性处理，但仍维持其部分结构和一些功能特性的产物。明胶作为胶原的衍生产物常用于包被(coating)细胞培养板或培养皿，以促进细胞的贴壁和增殖，广泛用于细胞培养。 本产品包被后的表面介质支持多种贴壁细胞培养，不仅能支持常规哺乳动物细胞的贴壁培养，还能用于小鼠胚胎干细胞的培养和传代，具有饲养层功能但丧失有丝分裂活性的小鼠胚胎成纤维细胞培养；以及人类胚胎多能干细胞、人诱导性多能干细胞来源的拟胚小体(Embryoid bodies)的多个胚层的定向分化等。 本产品用于E14小鼠胚胎干细胞的培养效果请参见图1。
图1.阿拉丁的UltraBio™ 0.1%明胶溶液(胚胎细胞培养级)用于E14小鼠胚胎干细胞的培养效果图。将E14小鼠胚胎干细胞单细胞悬液接种至UltraBio™ 0.1%明胶溶液(胚胎细胞培养级)包被后的6孔板，在无饲养层细胞支持下培养3天后的生长形态。Scale bar, 100μm。不同实验条件下的效果会有所差别，上图仅供参考。 对于6孔板，每孔推荐使用2ml，每100ml可用于50个孔；对于96孔板，每孔推荐使用100μl，每100ml可用于1000个孔。 UltraBio™ 1%明胶溶液（胚胎细胞培养级） 阿拉丁生产的UltraBio™ 1%明胶溶液(胚胎细胞培养级)，即 UltraBio™ 1% Gelatin Solution (Embryo cell culture tested)，是一种经测试可以直接用于胚胎干细胞培养的无菌高品质1%明胶溶液。本产品主要用于细胞培养板、培养皿等表面的包被(Coating)，以促进哺乳动物细胞的贴壁和增殖。 明胶是细胞外基质蛋白胶原(Collagen)经过不可逆变性处理，但仍维持其部分结构和一些功能特性的产物。明胶作为胶原的衍生产物常用于包被(coating)细胞培养板或培养皿，以促进细胞的贴壁和增殖，广泛用于细胞培养。 本产品包被后的表面介质支持多种贴壁细胞培养，不仅能支持常规哺乳动物细胞的贴壁培养，还能用于小鼠胚胎干细胞的培养和传代，具有饲养层功能但丧失有丝分裂活性的小鼠胚胎成纤维细胞培养；以及人类胚胎多能干细胞、人诱导性多能干细胞来源的拟胚小体(Embryoid bodies)的多个胚层的定向分化等。 本产品用于E14小鼠胚胎干细胞的培养效果请参见图1。
图1.阿拉丁的UltraBio™ 1%明胶溶液(胚胎细胞培养级)用超纯水稀释10倍后，用于E14小鼠胚胎干细胞的培养效果图。将E14小鼠胚胎干细胞单细胞悬液接种至 UltraBio™ 1%明胶溶液(胚胎细胞培养级)用超纯水10倍稀释后包被后的6孔板，在无饲养层细胞支持下培养3天后的生长形态。不同实验条件下的效果会有所差别，上图仅供参考。 对于6孔板，每孔推荐使用2ml 0.1%明胶溶液，每100ml本产品按照使用说明稀释10倍后可用于500个孔；对于96孔板，每孔推荐使用100μl 0.1%明胶溶液，每100ml本产品稀释10倍后可用于10000个孔。
明胶溶液在温度较低时会有固体颗粒析出的现象，使用前在25℃-55℃孵育至完全溶解后使用。 包被过程结束后，无须润洗，可直接接种细胞。 使用时注意无菌操作，避免微生物污染。 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。 为了您的安全和健康，请穿实验服并戴一次性手套操作。 UltraBio™ 0.1% Gelatin Solution (Embryo Cell Culture Grade) Aladdin's UltraBio™ 0.1% Gelatin Solution (Embryo Cell Culture Grade) is sterile and can be used directly for embryonic stem cell culture. This product is mainly used for coating the surface of cell culture plates and petri dishes to promote the adhesion and the proliferation of mammalian cells. Gelatin is the extracellular matrix protein collagen that has been irreversibly denatured, but still maintains part of its structure and some functional properties. It is extensively used to coat cell culture plates or dishes to promote cell adhesion and proliferation. The surface of culture vessels after coating with this product supports the adherent culture and passage of conventional mammalian cells as well as embryonic stem cells. The coated culture vessels can also be used for mouse embryonic fibroblast culture with feeder function but loss of mitostic activity, directed differentiation of multiple germ layers of human embryonic pluripotent stem cells, and human induced pluripotent stem cell-derived embryoid bodies. Please refer to Figure 1 for the cultured E14 mouse embryonic stem cells in 6-well plates coated with this product.
Figure 1. E14 mouse embryonic stem cells cultured in 6-well plates coated with the UltraBio™ 0.1% Gelatin Solution (Embryo Cell Culture Grade). Cell suspension was inoculated into a 6-well plate coated with this product and cultured for 3 days without feeder layer cell support. Scale bar, 100μm. This figure is for reference only, which may vary due to experimental conditions. We recommend using 2ml of this product per well for 6-well plates and 100μl per well for 96-well plates.
UltraBio™ 1% Gelatin Solution (Embryo Cell Culture Grade)
Aladdin's UltraBio™ 1% Gelatin Solution (Embryo Cell Culture Grade) is sterile and can be used directly for embryonic stem cell culture. This product is mainly used for coating the surface of cell culture plates and petri dishes to promote the adhesion and the proliferation of mammalian cells. Gelatin is the extracellular matrix protein collagen that has been irreversibly denatured, but still maintains part of its structure and some functional properties. It is extensively used to coat cell culture plates or dishes to promote cell adhesion and proliferation. Stored at 4℃ for up to 3 months, or at -20℃ for at least one year.. The surface of culture vessels after coating with this product supports the adherent culture and passage of conventional mammalian cells as well as embryonic stem cells. The coated culture vessels can also be used for mouse embryonic fibroblast culture with feeder function but loss of mitostic activity, directed differentiation of multiple germ layers of human embryonic pluripotent stem cells, and human induced pluripotent stem cell-derived embryoid bodies. Please refer to Figure 1 for the cultured E14 mouse embryonic stem cells in 6-well plates coated with this product.
Figure 1. E14 mouse embryonic stem cells cultured in 6-well plates coated with the UltraBio™ 0.1% Gelatin Solution (Embryo Cell Culture Grade). Cell suspension was inoculated into a 6-well plate coated with this product and cultured for 3 days without feeder layer cell support. Scale bar, 100μm. This figure is for reference only, which may vary due to experimental conditions. We recommend using 2ml of this product per well for 6-well plates and 100μl per well for 96-well plates.
Precautions: This product may precipitate at low temperatures and should be completely dissolved by incubating at 25℃-55℃ before use. After coating culture vessels with this product, cells can be inoculated directly into vessels without rinsing. This product should be handled aseptically to avoid microbial contamination. This product is for R&D only. Not for drug, household, or other uses. For your safety and health, please wear a lab coat and disposable gloves during the operation. |
名称和识别符
| 分子类型 | 未知 |
|---|
安全和危险性（GHS）
技术规格说明书
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 E2520479 |
分析证书 | 25-03-28 | G753837 |
| E2520493 |
分析证书 | 25-03-28 | G753837 |
| E2520478 |
分析证书 | 25-03-28 | G753837 |
| E2520480 |
分析证书 | 25-03-28 | G753837 |
| D2517447 |
分析证书 | 25-03-28 | G753837 |
| D2517448 |
分析证书 | 25-03-28 | G753837 |
| D2517435 |
分析证书 | 25-03-28 | G753837 |
| D2517451 |
分析证书 | 25-03-28 | G753837 |