| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| E665966-50ml |
50ml |
现货  |
| |
| E665966-250ml |
250ml |
期货 |
|
| 英文名称 | eECL Western Blot Kit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 储存温度 | 2-8°C储存,避光 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 运输条件 | 冰袋运输 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 产品介绍 |
产品内容
产品简介 高灵敏度化学发光检测试剂盒是免疫印迹实验中与辣根过氧化物酶（HRP）配套使 用的的高灵敏增强型检测试剂盒。该产品基于新一代增强型化学发光底物研制而成，在HRP的催化下发生化学反应而发光，可用于检测固定在膜上的蛋白质等生物大分子。其 高灵敏度能够检测pg级别的抗原，发光信号强烈持久，可以使用X-光胶片曝光或者化学 发光成像仪进行检测。 注意事项 1. 在与膜发生接触过程中，请佩戴手套且使用干净镊子等洁净器材，避免蛋白污染及高背景。 2. 避光条件下，配制好的化学发光检测底物工作液可在室温下稳定保存8小时。阳光或其他强 光会影响工作液，故应避免强光长时间的照射。正常实验室灯光短时间照射不影响工作液的 使用。 3.阿拉丁试剂提供多种蛋白转移用膜、封闭液、一抗、酶标二抗、缓冲液等，详情请查询公司网站。 操作步骤 1. 二抗孵育完毕后，将印迹膜充分洗涤。 2. 根据需要量，将eECL-A和eECL-B按照1：1的比例等体积混合，配制为化学发光检测底物工作液（一张8cm×6cm的膜大约使用1ml工作液）。 3. 弃去洗涤缓冲液，将发光底物工作液滴加在印迹膜上，确保工作液覆盖整张膜，室温孵育3-5分钟。 4. 用移液器吸去多余发光底物工作液，将印迹膜置于两层干净的塑料薄膜之间，此过程应小心完成，避免膜与膜之间产生气泡。 5. 在暗室内进行X-光胶片曝光，或将膜放置到化学发光成像仪内，按照仪器说明书进行检测。 附表
Product content
Products The High Sensitivity Chemiluminescent Assay is a highly sensitive and enhanced assay for use with horseradish peroxidase (HRP) in immunoblotting experiments. It is based on a new generation of enhanced chemiluminescent substrate, which is catalyzed by HRP to emit light, and can be used to detect biological macromolecules such as proteins immobilized on membranes. The high sensitivity enables the detection of pg-level antigens with a strong and long-lasting luminescence signal, which can be detected by X-ray film exposure or chemiluminescence imaging. Caveat 1. Please wear gloves and use clean equipment such as tweezers during the contact with the membrane to avoid protein contamination and high background. 2. Under the condition of avoiding light, the prepared working solution of chemiluminescence detection substrate can be stored stably at room temperature for 8 hours. Sunlight or other strong light may affect the working solution, so avoid prolonged exposure to strong light. Normal laboratory lighting for a short period of time does not affect the use of the working solution. 3. Aladdin Reagent offers a wide range of membranes for protein transfer, containment solutions, primary antibodies, enzyme-labeled secondary antibodies, buffers, etc. For details, please check the company's website. Procedure 1. After incubation of the secondary antibody, the blotting membrane is washed thoroughly. 2. According to the required amount, eECL-A and eECL-B are mixed in equal volumes at a ratio of 1:1, and prepared as a chemiluminescence detection substrate working solution (about 1m working solution is used for an 8cm×6cm membrane). 3. Discard the washing buffer, add the luminescent substrate working solution dropwise onto the blotting membrane, make sure the working solution covers the whole membrane, and incubate for 3-5 minutes at room temperature. 4. Pipette off excess luminescent substrate working solution and place the blotting membrane between two layers of clean plastic film, this process should be completed carefully to avoid air bubbles between the membrane and the membrane. 5. X-ray film exposure is performed in a dark room, or the membrane is placed inside a chemiluminescence imager and tested according to the instrument instructions. Schedules
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| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ25F0420894 |
分析证书 | 25-04-27 | E665966 |
| ZJ24F1114453 |
分析证书 | 24-11-27 | E665966 |
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