| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| C278962-150U |
150U |
期货  |
| |
| C278962-2000U |
2000U |
期货 |
|
| 产品名称 | 胶原酶 AF-1 来源于溶组织梭菌 |
|---|---|
| 英文别名 | Collagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene |
| 规格或纯度 | EnzymoPure™, ≥ 3.00 U/mg |
| 产品介绍 |
描述 胶原酶AF-1使用完全不含任何动物成分的生产工艺衍生自溶组织梭状芽胞杆菌。因此，排除了潜在的动物源性病原体传播的任何风险。这种高度纯化的胶原酶，通常与中性蛋白酶AF结合，适用于从几个组织中分离敏感细胞。已经证明，不含动物的酶对于从拟移植的人胰腺中分离朗格汉斯胰岛以及从脂肪组织和肿瘤细胞中分离基质血管级分（SVF）都是非常有效的。 应用 胶原酶AF-1可用于多种细胞分离。我们更大的包装规格为2000 U，是人类胰岛分离的理想选择，而较小的包装规格为150 U，可用于肿瘤细胞分离，从脂肪组织中分离TIL或SVF。 规格 活性≥3.00 U / mg *。 内毒素≤10.0 EU ** / mg（Ph.Eur。） 微生物学检查：总需氧微生物数（TAMC），总酵母/霉菌总数（TYMC），无梭状芽胞杆菌。 最终产品经过0.22 µm过滤。 （Clostridiopeptidase A，胶原酶A） EC 3.4.24.3 • Mr Mr 70 000 - 120 000 (collagenase) • CAS [9001-12-1] 参考和定义 *单位定义：胶原酶：1 U在25°C，pH 7.1下每分钟催化1 µmol 4-苯基偶氮苄氧基羰基-L-脯氨酰-L-亮氨酰甘氨酰-L-脯氨酰-D-精氨酸的水解。 **内毒素：欧洲药典 [1内毒素单位等于WHO批准的参考标准内毒素（RSE）的1国际单位]。 References 1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51 Collagenase AF-1 is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This highly purified collagenase, often in combination with Neutral Protease AF is suitable for the dissociation of sensitive cells from several tissues. The animal-free enzymes have been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation and stromal vascular fraction (SVF) from adipose tissue and tumor cells. Application Collagenase AF-1 can be used for several cell isolations. Our bigger packaging size of 2000 U is ideal for human islet isolation, whereas the smaller size of 150 U can be used for tumor cell isolation, isolation of TILs or SVF from adipose tissue. Specifications Activity (Wünsch) ≥ 3.00 U/mg*. Endotoxin ≤ 10.0 EU**/mg (Ph. Eur.) Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia. Endproduct is 0.22 µm filtered.
(Clostridiopeptidase A, Collagenase A) EC 3.4.24.3 • Mr Mr 70 000 - 120 000 (collagenase) • CAS [9001-12-1] References and Definitions *Unit definitions:Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1. **Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO appoved reference standard endotoxin (RSE)]. References 1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51 |
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
| 浓度 | ≥ 3.00 U/mg |
|---|---|
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| CAS编号和信息 | 9001-12-1 |
| 酶学委员会编号 | 3.4.24.3 |
| 单位定义 | 1 U在25°C，pH 7.1下每分钟催化1 µmol 4-苯基偶氮苄氧基羰基-L-脯氨酰-L-亮氨酰甘氨酰-L-脯氨酰-D-精氨酸的水解。 |
| 分子类型 | 小分子 |
| 1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke. (2023) Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma. BIOMEDICINE & PHARMACOTHERAPY, 165 (115096). [PMID:37421781] [10.1016/j.biopha.2023.115096] |
| 2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang. (2023) The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 242 (124820). [PMID:37178890] [10.1016/j.ijbiomac.2023.124820] |
| 3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu. (2023) A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 668 (131410). [10.1016/j.colsurfa.2023.131410] |
| 4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang. (2023) Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 631 (122489). [PMID:36521639] [10.1016/j.ijpharm.2022.122489] |
| 5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen. (2023) Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding. Materials Today Bio, 18 (100506). [PMID:36471892] [10.1016/j.mtbio.2022.100506] |
| 6. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng. (2022) In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration. Smart Materials and Structures, 31 (10): (105002). [10.1088/1361-665X/ac884a] |
| 7. Qian Ji, Hao Zhang, Xihe Zhang, Qiaoqiao Ma, Liping Teng, Lipeng Qiu. (2020) Hydrosoluble collagen based biodegradable hybrid hydrogel for biomedical scaffold. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 31 (17): (2199-2219). [PMID:32663418] [10.1080/09205063.2020.1796229] |
| 8. Guangxiu Cao, Cunyang Wang, Yubo Fan, Xiaoming Li. (2020) Biomimetic SIS-based biocomposites with improved biodegradability, antibacterial activity and angiogenesis for abdominal wall repair. Materials Science & Engineering C-Materials for Biological Applications, 109 (110538). [PMID:32228945] [10.1016/j.msec.2019.110538] |
| 9. Dajiang Kuang, Fujian Jiang, Feng Wu, Kulwinder Kaur, Sourabh Ghosh, Subhas C. Kundu, Shenzhou Lu. (2019) Highly elastomeric photocurable silk hydrogels. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 134 (838). [PMID:31103592] [10.1016/j.ijbiomac.2019.05.068] |
| 10. Huiyan Ying, Juan Zhou, Mingyu Wang, Dandan Su, Qiaoqiao Ma, Guozhong Lv, Jinghua Chen. (2019) In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing. Materials Science & Engineering C-Materials for Biological Applications, 101 (487). [PMID:31029343] [10.1016/j.msec.2019.03.093] |
| 11. Juan Zhou, Huiyan Ying, Mingyu Wang, Dandan Su, Guozhong Lu, Jinghua Chen. (2018) Dual layer collagen-GAG conduit that mimic vascular scaffold and promote blood vessel cells adhesion, proliferation and elongation. Materials Science & Engineering C-Materials for Biological Applications, 92 (447). [PMID:30184770] [10.1016/j.msec.2018.06.072] |
| 1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke. (2023) Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma. BIOMEDICINE & PHARMACOTHERAPY, 165 (115096). [PMID:37421781] [10.1016/j.biopha.2023.115096] |
| 2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang. (2023) The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 242 (124820). [PMID:37178890] [10.1016/j.ijbiomac.2023.124820] |
| 3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu. (2023) A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 668 (131410). [10.1016/j.colsurfa.2023.131410] |
| 4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang. (2023) Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 631 (122489). [PMID:36521639] [10.1016/j.ijpharm.2022.122489] |
| 5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen. (2023) Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding. Materials Today Bio, 18 (100506). [PMID:36471892] [10.1016/j.mtbio.2022.100506] |
| 6. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng. (2022) In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration. Smart Materials and Structures, 31 (10): (105002). [10.1088/1361-665X/ac884a] |
| 7. Qian Ji, Hao Zhang, Xihe Zhang, Qiaoqiao Ma, Liping Teng, Lipeng Qiu. (2020) Hydrosoluble collagen based biodegradable hybrid hydrogel for biomedical scaffold. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 31 (17): (2199-2219). [PMID:32663418] [10.1080/09205063.2020.1796229] |
| 8. Guangxiu Cao, Cunyang Wang, Yubo Fan, Xiaoming Li. (2020) Biomimetic SIS-based biocomposites with improved biodegradability, antibacterial activity and angiogenesis for abdominal wall repair. Materials Science & Engineering C-Materials for Biological Applications, 109 (110538). [PMID:32228945] [10.1016/j.msec.2019.110538] |
| 9. Dajiang Kuang, Fujian Jiang, Feng Wu, Kulwinder Kaur, Sourabh Ghosh, Subhas C. Kundu, Shenzhou Lu. (2019) Highly elastomeric photocurable silk hydrogels. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 134 (838). [PMID:31103592] [10.1016/j.ijbiomac.2019.05.068] |
| 10. Huiyan Ying, Juan Zhou, Mingyu Wang, Dandan Su, Qiaoqiao Ma, Guozhong Lv, Jinghua Chen. (2019) In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing. Materials Science & Engineering C-Materials for Biological Applications, 101 (487). [PMID:31029343] [10.1016/j.msec.2019.03.093] |
| 11. Juan Zhou, Huiyan Ying, Mingyu Wang, Dandan Su, Guozhong Lu, Jinghua Chen. (2018) Dual layer collagen-GAG conduit that mimic vascular scaffold and promote blood vessel cells adhesion, proliferation and elongation. Materials Science & Engineering C-Materials for Biological Applications, 92 (447). [PMID:30184770] [10.1016/j.msec.2018.06.072] |
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