| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab091004-10μl |
10μl |
现货  |
| |
| Ab091004-50μl |
50μL |
现货 |
| |
| Ab091004-100μl |
100μL |
现货 |
| |
| Ab091004-1ml |
1ml |
期货 |
|
| 产品名称 | beta Actin Antibody |
|---|---|
| 别名 | β-肌动蛋白抗体 | ACTB抗体 | Anti-beta Actin抗体 | β-Actin | beta 肌动蛋白抗体 |
| 英文别名 | A26C1A antibody | A26C1B antibody | ACTB antibody | ACTB_HUMAN antibody | Actin beta antibody | Actin cytoplasmic 1 antibody | Actin, cytoplasmic 1, N-terminally processed antibody | Actx antibody | b actin antibody | b-actin antibody | Beta cytoskeletal |
| 规格或纯度 | ExactAb™, Validated, Azide Free, High performance, 1.0mg/mL |
| 宿主种属 | 兔(Rabbit) |
| 特异性 | ACTB |
| 应用 | WB,Flow,IF,ICC,IHC |
| 种属反应性 | 人(Human),大鼠(Rat),小鼠(Mouse) |
| 预测反应种属 | 仓鼠(Hamster),兔(Rabbit),狗(Dog),猪(Pig),猫(Cat),绵羊(Sheep),蜜蜂(Bee),豚鼠(Guinea pig),鱼(Fish),鸡(Chicken) |
| 免疫原 | Synthetic MAP peptide derived from human beta-Actin: 1-200/375. |
| 阳性对照 | WB: NIH/3T3, C6, A549, Ramos, Jurkat, HeLa, MCF-7 lysates. IHC: Human cervical carcinoma tissue. ICC/IF: HeLa cells. |
| 偶联 | Unconjugated |
| 备注 | 本产品即将售完下架，替代产品为 Ab090999 |
| 抗体类型 | Primary antibody |
|---|---|
| 克隆类型 | 多克隆抗体 |
| Format | Whole IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 来源 | Serum |
| 物理外观 | Liquid |
| 储存缓冲液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% ProClin 300 and 50% Glycerol. |
| 防腐剂 | 0.03% ProClin 300 |
| 浓度 | 1.0mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 在 -20 °C 下保存一年。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
beta Actin Antibody (Ab091004) - Western Blot
All lanes: beta Actin Antibody (Ab091004) at 1/50000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
beta Actin Antibody (Ab091004) - IHC
Tissue: Human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded.
Antigen retrieval: Citrate buffer (0.01M, pH 6.0) boiling bathing for 15 minutes; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37℃ for 20 minutes.
Incubation: beta Actin Antibody (Ab091004) 1/1500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
beta Actin Antibody (Ab091004) - ICC/IF
Hela cells: 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with beta Actin Antibody (Ab091004) 1/100, 90 minutes at 37°C; Followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37°C for 90 minutes, DAPI was used to stain the cell nuclei.
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
| 应用名称 | 稀释比例 |
|---|---|
| WB |
1:5000-50000
|
| IHC |
1:500-1000
|
| Flow Cytometry | 1μg/Test For flow cytometric staining, the suggested use of this reagent is 1 µL per million cells in 100 µL staining volume or 1 µL per 100 µL of whole blood. |
| IF/ICC |
1:100
|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ24F0404301 |
分析证书 | 25-01-16 | Ab091004 |
| ZJ24F1215526 |
分析证书 | 24-12-17 | Ab091004 |
| ZJ24F1011819 |
分析证书 | 24-10-11 | Ab091004 |
| ZJ23F0700611 |
分析证书 | 24-04-25 | Ab091004 |
| ZJ23F0700610 |
分析证书 | 24-04-25 | Ab091004 |
¥269.90
¥139.90
¥199.90
¥188.90
¥188.90
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Wang RE, Hunt CR, Chen J, Taylor JS.. (2011) Biotinylated quercetin as an intrinsic photoaffinity proteomics probe for the identification of quercetin target proteins.. Bioorg Med Chem, 19 (16): (4710-4720). [PMID:21798748] [10.1016/j.bmc.2011.07.005] |
| 3. Angelo LS, Maxwell DS, Wu JY, Sun D, Hawke DH, McCutcheon IE, Slopis JM, Peng Z, Bornmann WG, Kurzrock R.. (2013) Binding partners for curcumin in human schwannoma cells: biologic implications.. Bioorg Med Chem, 21 (4): (932-939). [PMID:23294827] [10.1016/j.bmc.2012.12.008] |
| 4. Ohmori, H H, Toyama, S S and Toyama, S S.. (1992) Direct proof that the primary site of action of cytochalasin on cell motility processes is actin.. The Journal of cell biology, [PMID:1734024] |
| 5. Nakajima-Iijima, S S, Hamada, H H, Reddy, P P and Kakunaga, T T.. (1985) Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2994062] |
| 6. Ponte, P P, Ng, S Y SY, Engel, J J, Gunning, P P and Kedes, L L.. (1984) Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA.. Nucleic acids research, (10): [PMID:6322116] |
| 7. Hanukoglu, I I, Tanese, N N and Fuchs, E E.. (1983) Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of 3' non-coding regions.. Journal of molecular biology, (5): [PMID:6842590] |
| 8. Zhang, Suisheng S and 5 more authors.. (2002) Nuclear DNA helicase II/RNA helicase A binds to filamentous actin.. The Journal of biological chemistry, (4): [PMID:11687588] |
| 9. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801] |
| 10. Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10): [PMID:12853948] |
| 11. Stüven, Theis T, Hartmann, Enno E and Görlich, Dirk D.. (2003) Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes.. The EMBO journal, (3): [PMID:14592989] |
| 12. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 13. Holaska, James M JM, Kowalski, Amy K AK and Wilson, Katherine L KL.. (2004) Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane.. PLoS biology, [PMID:15328537] |
| 14. Giannakopoulos, Nadia V NV and 9 more authors.. (2005) Proteomic identification of proteins conjugated to ISG15 in mouse and human cells.. Biochemical and biophysical research communications, (21): [PMID:16139798] |
| 15. Procaccio, Vincent V and 14 more authors.. (2006) A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.. American journal of human genetics, [PMID:16685646] |
| 16. Jønson, Lars L and 8 more authors.. (2007) Molecular composition of IMP1 ribonucleoprotein granules.. Molecular & cellular proteomics : MCP, [PMID:17289661] |
| 17. Lu, Xiaoqing X and 5 more authors.. (2007) HGAL, a lymphoma prognostic biomarker, interacts with the cytoskeleton and mediates the effects of IL-6 on cell migration.. Blood, (15): [PMID:17823310] |
| 18. Lange, Martin M and 16 more authors.. (2008) Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex.. Genes & development, (1): [PMID:18765789] |
| 19. Kudryashov, Dmitri S DS and 10 more authors.. (2008) Connecting actin monomers by iso-peptide bond is a toxicity mechanism of the Vibrio cholerae MARTX toxin.. Proceedings of the National Academy of Sciences of the United States of America, (25): [PMID:19015515] |
| 20. Li, Long-Yuan LY and 14 more authors.. (2011) Nuclear ErbB2 enhances translation and cell growth by activating transcription of ribosomal RNA genes.. Cancer research, (15): [PMID:21555369] |
| 21. Mi, Lixin and 8 more authors.. (2011) Identification of potential protein targets of isothiocyanates by proteomics.. Chemical research in toxicology, (17): [PMID:21838287] |
| 22. Schmidt, Mathias V MV and 19 more authors.. (2011) Tumor suppressor down-regulated in renal cell carcinoma 1 (DRR1) is a stress-induced actin bundling factor that modulates synaptic efficacy and cognition.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:21969592] |
| 23. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 24. Rivière, Jean-Baptiste JB and 32 more authors.. (2012) De novo mutations in the actin genes ACTB and ACTG1 cause Baraitser-Winter syndrome.. Nature genetics, (26): [PMID:22366783] |
| 25. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 26. Li, Ming-Ming MM and 18 more authors.. (2013) ALKBH4-dependent demethylation of actin regulates actomyosin dynamics.. Nature communications, [PMID:23673617] |
| 27. Heisler, David B and 10 more authors.. (2015) ACTIN-DIRECTED TOXIN. ACD toxin-produced actin oligomers poison formin-controlled actin polymerization.. Science (New York, N.Y.), (31): [PMID:26228148] |
| 28. Mu, P P, Akashi, T T, Lu, F F, Kishida, S S and Kadomatsu, K K.. (2017) A novel nuclear complex of DRR1, F-actin and COMMD1 involved in NF-κB degradation and cell growth suppression in neuroblastoma.. Oncogene, (12): [PMID:28604741] |
| 29. Drazic, Adrian A and 12 more authors.. (2018) NAA80 is actin's N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility.. Proceedings of the National Academy of Sciences of the United States of America, (24): [PMID:29581253] |
| 30. Schrank, Benjamin R BR and 7 more authors.. (2018) Nuclear ARP2/3 drives DNA break clustering for homology-directed repair.. Nature, [PMID:29925947] |
| 31. Wiame, Elsa E and 6 more authors.. (2018) NAT6 acetylates the N-terminus of different forms of actin.. The FEBS journal, [PMID:30028079] |
| 32. Kwiatkowski, Sebastian S and 10 more authors.. (2018) SETD3 protein is the actin-specific histidine N-methyltransferase.. eLife, (11): [PMID:30526847] |
| 33. Wilkinson, Alex W AW and 21 more authors.. (2019) SETD3 is an actin histidine methyltransferase that prevents primary dystocia.. Nature, [PMID:30626964] |
| 34. Guo, Qiong Q and 9 more authors.. (2019) Structural insights into SETD3-mediated histidine methylation on β-actin.. eLife, (20): [PMID:30785395] |
首页
400-620-6333
